liquid detergent and alginate lyase for men

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Novel synovial fluid recovery method allows for ...- liquid detergent and alginate lyase for men ,ing a hole-punch with a 3/16 inch diameter. After absorbing the target liquid, a total of 35 mL of alginate lyase in H 2O (1 unit/mL concentrationx55.04 g/ mL; derived from Flavobacterium, SigmaeAldrich, A1603-100MG) was added, and the resultant mixture was vortexed briefly and incubated at …Characterization of a new endo-type polysaccharide lyase ...Dec 01, 2018·In this study, a new alginate lyase encoding gene, tsaly6A, has been cloned from Thalassomonas sp. LD5 and expressed in Escherichia coli. The alginate lyase TsAly6A belongs to the PL-6 and produced high yield of alginate trisaccharides (64.5%) with an endo-mode of action. Meanwhile, TsAly6A showed cold-adapted and metal ions-resisted properties.



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Dec 18, 2020·The dual role of the cystathionine gamma-lyase/hydrogen sulfide pathway in CVB3-induced myocarditis in mice. BIOCHEM BIOPH RES CO . 2009 Oct 23;388(3):595-600. (IF 2.985)

Alginate Trisaccharide Binding Sites on the Surface of β ...

Apr 02, 2019·β-lactoglobulin (BLG) is a promiscuous protein in terms of ligand interactions, having several binding sites reported for hydrophobic biomolecules such as fatty acids, lipids, and vitamins as well as detergents. BLG also interacts with neutral and anionic oligo- and polysaccharides for which the binding sites remain to be identified. The multivalency offered by these carbohydrate ligands is ...

Molecules | Free Full-Text | Extraction and Modification ...

Marine macroalgal (seaweed) polysaccharides are highly promising for next-generation applications in several industries. However, despite the reported comprehensive potential of these polysaccharides, commercial products are scarce on the market. Seaweed cultivations are increasing in number and production quantity, owing to an elevated global trend of utilization interest in seaweed.

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Effects of Tripalmitin and Saffiower Oil on the Content of ...

Jul 23, 1994·社団法人日本農芸化学会 The effects of feeding of saturated and unsaturated fatty acids on the contents of short-chain fatty acids (SCFAs) derived from bacterial fermentation in the colon were examined in rats. The concentration of lipids in the feces was also measured. Tripalmitin or samower oil at the concentration of 10% (w/w) in the diet with 5% (w/w) corn oil were used as the ...

Purification and Characterization of a Novel Alginate ...

These characteristics suggested that AlgA is an endolytic polyM-specific alginate lyase (EC 4.2.2.3). The products of alginate and polyM degradation by AlgA were purified and identified through fast protein liquid chromatography and electrospray ionization mass spectrometry, which revealed that AlgA mainly produced disaccharides, trisaccharides ...

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Occupational exposure as a painter was considered by previous Working Groups in 1988 and 2007 (IARC, 1989, 2010a). Since that time new data have become available, which have been incorporated in this Monograph, and taken into consideration in the present evaluation.

Alginate-based hydrogels as drug delivery vehicles in ...

Mar 13, 2020·Chemical structure. Alginate (ALG) is composed of the irregular blocks of β-D-mannuronic acid (M) and 1–4 linked α-L-guluronic residues (G) which is the water-soluble linear polysaccharide.Its block-like structure is organized in the pattern of homogenous (poly-G, poly-M) or heterogeneous (MG) pattern [].Because of the specific profiles of the monomers and their modes of …

Characteristics and applications of alginate lyases: A ...

Dec 01, 2020·Alginate is a widespread polysaccharide composed of two isomer residues, β-D-mannuronic acid (M) and α-L-guluronic acid (G) [].These two residues are linked homogeneously or heterogeneously by 1 → 4 glycosidic bonds, which contributes to three different blocks in alginate, polyM blocks, polyG blocks and polyMG blocks [].Alginate is widely found in brown algae, and its content is ...

Purification and characterization of a novel alginate ...

Sep 16, 2019·A novel alginate lyase, named as AlgA, with high specific activity was purified from the culture medium of this strain. AlgA had a molecular weight of approximately 60 kDa, wtih the optimal temperature and pH of 40 C and 7.5,respectively.

Frontiers | Biotechnology Potential of Marine Fungi ...

However, their enzymatic profile included about 25–37 U/mg activities of agarase, alginate lyase, carragenase and fucoidanase during the first 4 days of cultivation dropped almost to zero at the following 7 days, probably, due to the growth on the plant substrate (Balabanova et al., 2018).

Functional Exploration of the Polysaccharide Lyase Family ...

Jul 20, 2016·Alginate, the main cell-wall polysaccharide of brown algae, is composed of two residues: mannuronic acid (M-residues) and, its C5-epimer, guluronic acid (G-residues). Alginate lyases define a class of enzymes that cleave the glycosidic bond of alginate by β-elimination.

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Biobased liquid laundry detergent. Signature scent features magnolia, bergamot, and cedar and is free of all EU listed fragrance allergens. Features eco-friendly Phytolase cleaning technology: a unique enzyme-driven formula to help break down tough stains and strong odors. Nontoxic, biobased formula that is readily biodegradable.

Purification and Characterization of a Novel Alginate ...

Purification and Characterization of a Novel Alginate Lyase from the Marine Bacterium Bacillus sp. Alg07 Peng Chen, Yueming Zhu, Yan Men, Yan Zeng, Yuanxia Sun; Affiliations Peng Chen National Engineering Laboratory for Industrial Enzymes, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, China ...

Alginate Trisaccharide Binding Sites on the Surfaceof β ...

The multivalency offered by these carbohydrate ligands is expected to facilitate coacervation, an electrostatically driven liquid–liquid phase separation. Using heteronuclear single quantum coherence NMR spectroscopy and monitoring chemical shift perturbations, we observed specific binding sites of modest affinity for alginate ...

OCCUPATIONAL EXPOSURE AS A PAINTER - Chemical Agents and ...

Occupational exposure as a painter was considered by previous Working Groups in 1988 and 2007 (IARC, 1989, 2010a). Since that time new data have become available, which have been incorporated in this Monograph, and taken into consideration in the present evaluation.

Alginate Lyase - an overview | ScienceDirect Topics

Takao Ojima, in Marine Enzymes for Biocatalysis, 2013. 13.2.4 Application of molluscan alginate lyase. Molluscan alginate lyases were used for the production of protoplasts from brown seaweed (Butler et al., 1989; Boyen et al., 1990).Recently, the use of recombinant abalone alginate lyase was shown to give a better yield of protoplast than the use of native enzyme prepared from hepatopancreas ...

Purification and Characterization of a Novel Alginate ...

These characteristics suggested that AlgA is an endolytic polyM-specific alginate lyase (EC 4.2.2.3). The products of alginate and polyM degradation by AlgA were purified and identified through fast protein liquid chromatography and electrospray ionization mass spectrometry, which revealed that AlgA mainly produced disaccharides, trisaccharides ...

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The role of bacterial biofilms in chronic infections ...

The development of in vitro biofilms by wild-type P. aeruginosa does not seem to involve the production or a dependence on alginate as a matrix component 81. This is probably an artefact of in vitro systems, because wild-type, non-mucoid P. aeruginosa produce small amounts of alginate when infecting experimental animals 82.

Kinetic and thermodynamic properties of alginate lyase …

May 01, 2017·The standard reaction mixture of alginate lyase consisted of 0.1 mL of the enzyme preparation (described above in Section 2.3), 0.1 mL of 50 mM Trizma™ buffer (pH 7.5) and 2.2 mL of 0.5 mg/mL (w/v) sodium alginate in the same buffer, was incubated for 20 min at 40 °C.

Isolation, identification, and whole genome sequence ...

Jul 02, 2020·Alginate-degrading bacteria or alginate lyases can be used to oligomerize alginate. In this study, an alginate-degrading bacterium with high alginolytic activity was successfully screened by …

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Process Biochemistry - Academic Accelerator

Process Biochemistry is an application-orientated research journal devoted to reporting advances with originality and novelty, in the science and technology of the processes involving bioactive molecules and living organisms. These processes concern the production of useful metabolites or materials, or the removal of toxic compounds using tools ...

Lyase from the Marine Bacterium Bacillus

2.2. Purification of Alginate Lyase from Bacillus sp. Alg07 Bacillus sp. Alg07 was cultured in optimized liquid medium for 24 h until its alginate lyase reached the highest activity. The supernatant containing 510 U/mL of alginate lyase was subjected to further purification through the two simple steps of tangential flow filtration ...

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